Figure 6.

OVA-specific T cell responses were promoted in Fcer1g^−/− mice. (A,B) Six-day-cultured BMDCs derived from WT and Fcer1g^−/− mice were pulsed with OVA323-339 peptide for 3 h, then CD4⁺ OT-II T cells isolated from OT-II transgenic mice were cultured with these BMDCs at indicated ratios for 3 days. The proliferation of T cells was determined by [³H]thymidine incorporation (A) and IL-17 production was detected by ELISA (B). (C,D) WT and Fcer1g^−/− mice were immunized with OVA (50 µg) mixed with incomplete Freund’s adjuvant and dZym (left panel) or curdlan (right panel) via footpads. After 7 days, the draining lymph nodes were isolated and total cells were collected. (C) The numbers of CD3⁺ cell were determined by counting and flow cytometry. (D) Total cells were cultured in 96-well plates with the indicated amounts of OVA for 3 days. The proliferation of T cells was measured by [³H]thymidine incorporation. Error bars indicated mean + SD of three independent experiments. The significances *p < 0.05, **p < 0.01 (Student’s t-test) were obtained by comparing Fcer1g^−/− to WT dendritic cells. All data shown are representative from three independent experiments.