Figure 8.

Phosphatases were recruited by FcRγ and negatively regulated Dectin-1 responses in DCs. Six-day-cultured BMDCs derived from WT mice were collected and treated with dZym. (A) Cell lysates were collected at indicated time points and incubated with anti-FcRγ Ab. After 16 h, protein A/G beads were added for precipitation. The phosphatases SHIP-1, SHP-1, SHP-2, and PTEN in whole cell lysate or associated proteins with FcRγ were detected by SDS-PAGE and Western blot. Quantification was determined by densitometry using ImageJ software. (B) BMDCs were incubated with sodium stibogluconate (SS) (SHP-1 inhibitor) or SF1670 (PTEN inhibitor) for 1 h before dZym treatment. After 16 h, the expressions of CD80, CD86, and MHC-II were detected by flow cytometry. The changes of MFIs from control to treatment are indicated in each histogram. Gray areas represented the isotype-matched Ig controls. All data shown were representative from three to five independent experiments.