Figure 3.

The differential expression of Sertoli and granulosa cell-specific genes in Ctnnb1-overactivated testes was performed with quantitative real-time PCR at E16.5. A, the expression of granulosa cell-specific genes was significantly increased in Ctnnb1-overactivated testes. B, the expression of Sertoli cell-specific genes was significantly decreased in Ctnnb1-overactivated testes. Gonads with the same genotype at each time point were pooled for RNA preparation. The experiments were performed with three independent pools. Data are presented as the mean ± S.E. (t test). *, p < 0.05; **, p < 0.01; NS, not significant.