Table 3.
Oligonucleotide primer sequences
| Name | Sequence | Use |
|---|---|---|
| LpoA(33)_F | CATGCCATGGCGAATTTCACGCAAACCTTACAA | PCR |
| LpoA(253)_R | GCCGACGTCGACTTGTTGGAAATTAAGCAATGTAAG | PCR |
| LpoA(573)_R | GCCGACGTCGACAACTGGTACAATTGCACCATC | PCR |
| F_LIC_yraM(29) | TACTTCCAATCCAATGCACTACTTGGTAGCAATTTCACG | PCR |
| R_LIC_STP_yraM(575) | TTATCCACTTCCAATGTTAGTTTGCAACTGGTACAATTG | PCR |
| LpoA_delH6_F | GATGGTGCAATTGTACCAGTTGCCAACTAACACCACCAC | QuikChange |
| LpoA_delH6_R | GTGGTGGTGTTAGTTGGCAACTGGTACAATTGCACCATC | QuikChange |
| pETBlueUp | TCACGACGTTGTAAAACGAC | Sequencing |
| pETBlueDOWN | GTTAAATTGCTAACGCAGTCA | Sequencing |
| nt746 | CAGCGAATACTGGCG | Sequencing |
| nt1139 | GGCCCATTACTAAAACAA | Sequencing |
| nt1520 | GCAGAAATGAAAGGTTAT | Sequencing |