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. 2017 Sep 8;292(43):17626–17642. doi: 10.1074/jbc.M117.804997

Table 4.

Bacterial strains and plasmids

Name Description/Protein expressed Parent plasmid/PCR primers Source/Reference
Strains
    H. influenzae Rd Source of genomic DNA Ref. 50
    NovaBlue Novagen
    OrigamiTM(DE3)/pLacI Expression host strain Novagen
    TunerTM(DE3)/pLacI Expression host strain Novagen
Expression plasmids
    pETBlue-2TM T7 promoter expression Novagen
    pLpoAN-His6 MA-LpoA(33–253)-VE-His6 pETBlue-2/LpoA(33)_F, LpoA(253)_R This work
    pLpoA-His6 MA-LpoA(33–573)-VE-His6 pETBlue-2/LpoA(33)_F, LpoA(573)_R This work
    pLpoA MA-LpoA(33–575) pLpoA-His6/LpoA_delH6_F, LpoA_delH6_R This work (primers for QuikChange mutagenesis)
    pMCSG7 T7 promoter expression, LIC pET21a Ref. 51, kind gift of Dr. Mark Donnelly
    pMCSG7-LpoA(29–575) His6-(TeV_site)-NA-LpoA (residues 29–575) pMCSG7/F_LIC_yraM(29), R_LIC_STP_yraM(575) This work (expressed protein for SAXS measurements)