Table 2.

ATP-induced pyrene(acto)·MYO1C dissociation by global fit simulation

Conditions used were: 20 mm MOPS, pH 7.0, 25 mm KAc, 2 mm MgCl₂, 0.2 mm EGTA, and 1 mm DTT at 20 ± 0.1 °C.

Constant	MYO1C35	MYO1C16	MYO1CC
k′+α (s−1)	0.50	0.86	1.20
k′−α (s−1)	0.17	0.58	1.60
K′α	2.99	1.48	0.75
K′1T k′+2T (μm−1 s−1)a	0.33	0.24	0.19
k′+1T (μm−1s−1)	6.25	5.13	4.34
k′−1T (s−1)	548.00	568.00	531.00
K′1T (mm−1)	11.41	9.03	8.17
k′+2T (s−1)	29.00	26.80	23.00
k′−2T (s−1)	7.79	3.97	0.97
K′2T	3.72	6.75	23.66
k′Diss (s−1)	5.08	5.71	1.02

^a Calculated.