2D 13C-13C INADEQUATE spectra of membrane-bound BM2(1–33) measured at 243 K.
A, 2D spectra at pH 5.5 and pH 7.3. B, His-27 chemical shifts as a function of pH. The Ser-9, Ile-14, Leu-18, and Ala-22 chemical shifts are insensitive to pH, whereas His-27 shows pH-dependent changes of Cα and Cβ chemical shifts.