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CPEB2A and CPEB2B act to either enhance or decrease HIF1α and TWIST1 levels via an increase in nascent protein levels. A, shows the input of either CPEB2A or CPEB2B-FLAG after labeling but prior to immunoprecipitation. B, click chemistry was used to label nascent proteins in MDA-MB-231 cells ectopically expressing either CPEB2A or CPEB2B. Biotin-labeled proteins were then precipitated using streptavidin beads and proteins thus isolated were probed using the indicated antibodies.
