Figure 5.

CPEB2A and CPEB2B regulate the metastasis of TNBC cells in an opposing fashion via TWIST1. A—D, MDA-MB-231 cells stably expressing CPEB2B were transduced with lentivirus to stably express shRNA directed toward TWIST1 or HIF1α. 250,000 cells were implanted into the T1 fat pad of NOD scid gamma mice, then primary tumors were all allowed to reach roughly 2000 mm³. Tumors were dissected out and measured (D) (black bar = 1 cm), lungs were harvested (B), gross lesions were counted (A, and shown by arrows in B), and lungs were subjected to H&E staining (C). D, lungs were also subjected to sectioning followed by H&E staining. Scale bar indicates the following lengths according to magnification: 4×, 1.0 mm; 20×, 200 μm. * = p < 0.05 compared with controls. # = p < 0.05 compared with rescue.