Figure 4.

miR-215 reduces migration and invasion ability of HCC cells. Overexpression of miR-215 or two PCAT-1 siRNAs (siPCAT-1-1 and siPCAT-1-2) inhibits wound-healing in SMMC7721 and BEL7402 cells. However, suppression of miR-215 accelerates wound healing in SMMC7721 and BEL7402 cells. A and B, wound fields were observed directly after removal of inserts (0h) and cell migration was followed for 36 and 72 h in SMMC7721 cells (A) or for 36 and 72 h in BEL7402 cells (B). The wound-healing area in HCC cells is presented by a histogram. C, miR-215 inhibits the invasion abilities of SMMC7721 and Huh7 cells. Cells on the lower surface of the chamber were stained by crystal violet at 48 h after transfection. *, p < 0.05; **, p < 0.01; ##, p < 0.01.