Figure 3.

Analysis of proMMP-9 maturation kinetics by gelatin zymography in the presence and absence of 10 μm JNJ0966. A, gelatin zymogram of proMMP-9 samples that have been reacted with catMMP-3 in the presence and absence of 10 μm JNJ0966 for 0, 10, 20, 30, 40, 50, and 60 min. Zymograms are duplicate pictures; the bottom panel is overlaid with graphical lines to illustrate the three different MMP-9 molecular species (92, 86, and 82 kDa). B–D, graphs of relative abundance of the individual MMP-9 forms as a percentage of total enzyme abundance in each condition as measured by densitometric quantitation of the zymogram. In all graphs, relative abundance of proMMP-9 species is depicted from DMSO control (red lines) and 10 μm JNJ0966 (blue lines) conditions. B, graph of the unprocessed 92-kDa proMMP-9 with (blue lines) and without (red line) JNJ0966. C, graph of the 86-kDa intermediately processed proMMP-9. D, graph of the 82-kDa fully processed MMP-9.