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. 2017 Oct 31;7:14398. doi: 10.1038/s41598-017-14719-9

Figure 1.

Figure 1

Abundance of calpain protein and its activity was increased in obese adipose tissue. (A) Calpain-1, -2, -4, CAST and β-actin protein were detected by Western blotting in epididymal white adipose tissue (EpiWAT) extracts of wild type mice fed either a LFD or HFD for 16 weeks (n = 3). (B) Cross-sections from the EpiWAT of LFD and HFD fed wild type mice were immunostained with anti-mouse calpain-2 (red color). Arrows indicate positive immunostaining. (C) Calpain activity was measured by fluorimetric assay in EpiWAT from LFD and HFD fed mice (n = 4). (D) Calpain-1, -2 and -4 protein were detected by Western blotting in matured adipocyte and SVF cells. CAST protein was detected in EpiWAT (E) and BMDM. (F) Representative Western blot images are cropped from the full-length blot. The full-length Western blots are included in the Supplementary information. (G) Calpain activity was measured by fluorimetric assay in EpiWAT from LFD and HFD fed WT and CAST Tg mice (n = 4). (H) Body weight of LFD and HFD fed male WT and CAST Tg mice (n = 13–15). Body fat mass (I) and lean mass (J) of LFD and HFD fed male WT and CAST Tg mice (n = 12–14). *And horizontal lines represent significance of P < 0.05 (Two-way ANOVA with Holm-Sidak post hoc analysis).