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. 2017 Oct 31;7:14398. doi: 10.1038/s41598-017-14719-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CAST overexpression reduced interstitial fibrosis in adipose tissue. (A) Representative Sirius red staining of EpiWAT cross-sections from LFD and HFD fed CAST WT and Tg mice. (B) mRNA abundance of Col I, Col III, Col IV, Col V and Col VI genes in EpiWAT from LFD and HFD fed CAST WT and Tg mice were analyzed by qPCR (n = 5). Values are represented as mean ± SEM. *and # denotes P < 0.05 when comparing LFD vs HFD and WT vs Tg respectively (Two-way ANOVA with Holm-Sidak post hoc analysis).