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. 2017 Oct 31;7:14416. doi: 10.1038/s41598-017-14882-z

Figure 1.

Figure 1

DMB expression associated with HTLV-1 infection. (A) MT2, C8166 and Jurkat cells were lysed and analyzed with an anti-DMΒ antibody. (B) Hela cells (top) or PMA-THP1 cells (bottom) were co-cultured with MT2 cells for indicated time. Afterwards, the cells were washed with PBS three times to remove MT2 cells and lysed for immunoblot assays. (C) Hela cells were transfected with SC or indicated siRNA. At 24 h after transfection, the cells were co-cultured with MT2 cells for another 24 h. Then the cells were washed with PBS three times to remove MT2 cells and lysed for immunoblot analyses. (D) Hela cells were treated with DMSO, Fludarabine (10 uM), Nifuroxazide (25 ug/mL), BAY11-7082 (10uM), SB203580 (5 uM), SP600125 (10uM) or U0126 (1 uM) and co-cultured with MT2 cells for 18 h. Then the cells were washed with PBS three times to remove MT2 cells and lysed for immunoblot analyses. β-actin was used as a loading control in the immunoblot assays. The data were representative of three independent experiments.