Figure 6.

DMB interacts with ATG7. (A) HEK293T cells were transfected with indicated plasmids. The cell lysates were immunoprecipitated (IP) with anti-HA and immunoblotted (IB) with anti-HA or anti-DMB as indicated. (B) HEK293T cells were transfected with HA-tagged ATG7 and empty vector or pcDNA3.1-DMB. The cell lysates were immunoprecipitated (IP) with anti-DMB and immunoblotted (IB) with anti-DMB or anti-HA as indicated. (C) MT2 cells were immunoprecipitated with anti-ATG7 and immunoblotted with anti-DMB or anti-ATG7 as indicated. (D) PMA-THP1 cells were co-cultured with MT2 cells for 24 h. The cell lysates were then immunoprecipitated with anti-ATG7 and immunoblotted with anti-DMB or anti-ATG7 as indicated. (E) Confocal microscopy assays of Hela cells transfected with Cherry-ATG7 (Red) and EGFP-DMB (Green). The data were representative of three independent experiments.