Figure 8.

The effects of DMB on the acetylation of ATG7. (A) Hela cells were transfected with indicated plasmids for 24 h, and then co-cultured with or without MT2 cells for another 12 h. The cells were washed with PBS three times to remove MT2 cells and lysed. The cell lysates were immunoprecipitated with anti-HA and immunoblotted with anti-Acetylated-Lysine antibody or anti-HA as indicated. (B) MT2 cells were transfected with SC or H3. At 24 h after transfection, the cell lysates were immunoprecipitated with anti-ATG7 or immunoblotted with anti-Acetylated-Lysine antibody or anti-ATG7 as indicated. (C) Hela cells were transfected with indicated GFP-tagged plasmids for 24 h, and then co-cultured with or without MT2 cells for another 12 h. The cells were washed with PBS three times to remove MT2 cells and lysed. The cell lysates were immunoprecipitated with anti-ATG7 and immunoblotted with anti-Acetylated-Lysine antibody or anti-ATG7 as indicated. (D) PMA-THP1 cells were transfected with SC or H3 for 24 h, and then co-cultured with MT2 cells for another 24 h. The cells were washed with PBS three times to remove MT2 cells and lysed. The cell lysates were immunoprecipitated with anti-ATG5 and immunoblotted with the indicated antibodies. (E) MT2 cells were transfected with SC or H3. At 24 h after transfection, the cells were lysed. The cell lysates were immunoprecipitated with anti-ATG5 and immunoblotted with the indicated antibodies. β-actin was used as a loading control in the immunoblot assays. The data were representative of three independent experiments. (F) The schematic diagram of the interactions among HTLV-1, autophagy and DM. ①HTLV-1 infection induced the accumulation of autophagosome and this accumulation enhanced HTLV-1 protein expression. ②Meanwhile, HTLV-1 infection induced the expression of DMB. ③DMB expression increased the acetylation of ATG7, leading to decreased autophagy accumulation and lower HTLV-1 protein expression levels. Ac, acetylation.