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. 2017 Oct 31;7:14432. doi: 10.1038/s41598-017-14644-x

Figure 1.

Figure 1

Mean fertilization success of A. tenuis (n = 2 egg donors and 4 to 6 pooled sperm donors, 22 to 75 eggs/treatment) and A. millepora (n = 2 egg donors and n = 4 to 6 pooled sperm donors, 14 to 114 eggs/treatment), using a 5 ml in vitro system. The fertilization success for both species was pooled and the relative success of the five treatments examined. The five treatments included no sperm added, fresh sperm, no freezing (sperm just exposed to 10% DMSO) and sperm exposed to 10% dimethyl sulfoxide and cryopreserved for less than 1 month and over 1 to 2 yr (striped bar). Bars represent means (±SE) and different letters indicated differences in the mean (p < 0.05, ANOVA, F = 17.34).