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. 2017 Sep 29;11(11):1595–1615. doi: 10.1002/1878-0261.12134

Figure 6.

Figure 6

LacdiNAc is expressed in ovarian cancer cells and absent in normal surface epithelium as well as primary peritoneal cancer cells. (A) Hypothetical synthesis pathway of LacdiNAc structures on N‐glycoproteins. (B) Differentially expressed genes encoding glycosyltransferases involved in synthesis of LacdiNAc structures (B4GALNT3 and B4GALNT4) and expression of sialyltransferase genes (ST6GAL2,ST3GAL4 and ST3GAL5) using transcriptomic data obtained from ovarian‐derived (OV) and peritoneum‐derived (PE) cancer tissues (Tothill data set). Adjusted P‐values are shown in each box plot. (C) Extraction ion chromatogram to show expression of LacdiNAc structures (m/z 1205.02− and m/z 1059.42−) in normal epithelial cell lines (HOSE6‐3, HOSE17‐1), ovarian cancer cells [OVCAR3, SKOV3, IGROV1, A2780 and EFO27 (shift in retention time)] and primary peritoneal cells (S129). (D) Bar graph shows relative gene expression of relevant glyco‐genes among the tested cell lines. Gene expression is displayed relative to the cell line with lowest expression.