Figure 6.

Propofol does not induce apoptosis in neural stem cells (NSCs) in hippocampus. (A) Fluorescence images of hippocampus treated with propofol. The brain sagittal sections were co-stained with activated caspase 3 and nestin (NSC marker). Cell nuclei were stained with Hoechst 33342. Blue, green, and red represent cell nuclei, nestin, and activated caspase 3 signals, respectively. Scale bar = 100 µm; (B) The magnified view of the yellow-boxed region marked in Figure 6A. These images include the individual channel and overlaid images and show that activated caspase 3 and nestin signals were not co-localized in the same cells, suggesting that propofol does not induce the apoptotic NSC death. Scale bar = 20 µm.