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. 2017 Oct 21;9(10):139. doi: 10.3390/cancers9100139

Figure 6.

Figure 6

Humanized CD47 CAR-T cells are highly cytotoxic against CD47-positive cancer cells. (A) RTCA cytotoxicity assay with humanized CD47-CAR-T cells and CD47-positive or CD47-negative Hela-CD19 cancer target cells. CD47-CAR-T cells cytotoxic against CD47-positive but not against CD47-negative Hela-CD19 cells. Positive control CD19-CAR-T cells effectively kill CD19-positive Hela-CD19 cells; (B) Quantification of RTCA assay show significantly increased CD47-CAR-T cell cytotoxicity with CD47-positive cancer cell lines but not with CD47-negative Hela-CD19 cells. * p < 0.001, CD47-CAR-T cells versus Mock-CAR-T cells, Student’s t test. Bars show average percent of cytotoxicity ± standard deviations in three independent experiments; (C) Increased secretion of IL-2 by humanized CD47-CAR-T cells with CD47-positive cells SKOV-3 cells. E:T ratio=1:1. p < 0.05, CD47-CAR-T cells versus controls, Student’s t-test, n = 3; (D) IL-2 is not secreted by CD47-CAR-T cells with CD47-hegative cells, Hela-CD19 cells. Mock-CAR-T cells are negative control cells. CD19-CAR-T cells are positive control cells against Hela-CD19 target cells. E:T ratio=10:1. p < 0.05, CD19-CAR-T cells versus controls, Student’s t-test, n = 3.