Figure 4.
Slug mediates the expression level of CDD and hENT1 in PDAC. Representative immunoblots (A) and (B) show the expression of Slug, hENT1 and CDD in AsPC-1 and BxPC-3 after Slug-specific siRNA treatment. 3 × 105 cancer cells were seeded in 6-well plate for 24 h. Slug siRNAs (the number #1 and #2 designate two different Slug siRNA sequences) or a negative control siRNA (75 nM) in serum/antibiotic-free media were added to the adherent cells and incubated for 24 h. The media was then replaced with fresh media (+10% fetal bovine serum (FBS)) and cells incubated for a further 24 h before cells were lysed and examined for the expression of the proteins of interest. Bar graphs (C) and (D) show the expression of Slug, hENT1, CDD and loading control α-actinin in AsPC-1 and BxPC-3 post siRNA treatment. The expression levels were quantified relative to the loading control. The columns represent the fold change of protein levels relative to the negative control siRNA treated cells (n ≥ 3). Data are presented as mean ± SEM. One way ANOVA with post-hoc Bonferroni’s Multiple Comparison Test was used to examine the significance of the mean *** p < 0.0001, ** p < 0.001, * p < 0.05. Neg. Cont.: negative control.