FIGURE 3.

Heterologous expression of eight CsSWEET genes in yeast. Yeast strains with recombinant vectors or the empty pDR196 vector (as a negative control) were grown on SD (synthetic deficient)-ura medium supplemented with 2% maltose or different sugars (listed above) as the sole carbon source for 5 days. Medium with maltose as sole carbon source is a positive control for strain EBY.WV4000, while with glucose as sole carbon source is a positive control for strain SUSY7/ura. Yeast cell suspensions were diluted (×10, ×10², ×10³) for serial dilutions assay. The other results of the experiment are in Supplementary Figure S2.