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. 2017 Sep 26;8(10):241. doi: 10.3390/genes8100241

Figure 3.

Figure 3

Observation of fungal development in rice CO-39 leaves. (A) Fungal development in rice CO-39 leaves was observed by confocal microscope. Plates represent a projection of scans taken from inside Leaf 6 of rice plants 10 days after M. oryzae infection but previously inoculated with BMV carrying derivatives of recombinant RNA3 vector expressing target MoABC1, MoMAC1 and MoPMK1 gene segments in as a mixture of sense plus antisense form. WT, Mock and BMV (without inserting) plants were used as controls. Silencing of the targeted M. oryzae genes induced by host-generated RNAi restricts fungal development whereas control plants show extensive mycelia growth. Scale bars are 10 µm. (B) Under a scanning electron microscope (SEM), fungal development in rice leaves inoculated with BMV variants were observed. Scale bars are 50 µm.