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Minigene splicing assay of Wnt family member 10A (WNT10A) variants c.374G>A and c.125A>G. (A) Agarose gel electrophoresis of PCR products. No abnormal splicing product was found in either HeLa or HEK-293T cells. (B–D) Sequencing results of (B) the wild-type minigene, (C) the c.125A>G minigene, and (D) the c.374G>A minigene. The minigenes containing variant c.374G>A or c.125A>G spliced normally.
