Table 3.
List of samples containing Larix sequences in the metabarcoding data, with their extraction negative controls (EN), sorted by the core or hand-pieces (HP) from which they were collected. PCR and cloning success (+: successful, −: not successful) are given, as is the number of retrieved sequences for each sample and the detected SNP variants of the two markers cp77444 and cp103595 including their abundance. If PCRs were not successful, the samples were not cloned (n.c.).
| Core/HP | Sample Depth | PCR-Product cp77444 | PCR-Product cp103595 | Cloning Success cp77444 | Cloning Success cp103595 | No. of Retrieved Sequences cp77444 | Detected Variants cp77444 | No. of Retrieved Sequences cp103595 | Detected Variants cp103595 |
|---|---|---|---|---|---|---|---|---|---|
| L14-02 | 7.53 m | + | + | + | - | 0 | - | - | - |
| - | EN2 | - | - | n. c. | n.c. | - | - | - | - |
| L14-03 | 6.72 m | + | + | + | + | 0 | - | 13 | T |
| - | EN3 | - | - | n.c. | n.c. | - | - | - | - |
| L14-03 | 7.59 m | + | - | + | n.c. | 11 | A | - | - |
| L14-03 | 8.56 m | + | + | + | + | 8 | A | 7 | 3× C, 4× T |
| L14-03 | 9.61 m | - | - | n.c. | n.c. | - | - | - | - |
| L14-03 | 10.11 m | - | - | n.c. | n.c. | - | - | - | - |
| L14-03 | 11.77 m | + | + | + | + | 9 | A | 3 | T |
| - | EN5 | - | - | n.c. | n.c. | - | - | - | - |
| L14-05 | 3.3 m | - | + | + | + | 1 | A | 1 | T |
| L14-05 | 3.7 m | - | - | n.c. | n.c. | - | - | - | - |
| - | EN6 | - | - | n.c. | n.c. | - | - | - | - |
| L14-04 | 6.15 m | - | + | n.c. | + | - | - | 2 | C |
| L14-04 | 7.27 m | + | + | + | + | 7 | A | 13 | T |
| - | EN7 | - | - | n.c. | n.c. | - | - | - | - |
| L14-04 | 8.03 m | + | + | + | + | 7 | 3× C, 4× A | 7 | 1× C, 6× T |
| L14-04C | 2.05 m | + | + | + | + | 9 | A | 11 | C |
| L14-04C | 3.15 m | + | + | + | + | 10 | A | 15 | 8× C, 7× T |
| L14-04C | 3.55 m | + | + | + | + | 6 | A | 13 | 2× C, 11× T |
| - | EN8 | - | - | n.c. | n.c. | - | - | - | - |
| - | NTCs | - | - | n.c. | n.c. | - | - | - | - |