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The 1°DKIEs determined for dianion activated reduction of GA catalyzed by GPDH that were determined from the fit of kinetic data to the mechanism shown in Scheme 9.78 Essentially the same disassociation constants KX and KGA were obtained from the fit of data for the reactions of NADH and NADD, so that the 1°DKIE on the kinetic parameters kcat, kcat/KGA and kcat/KGAKX for the different dianion activated reactions are equal to the quoted values of kH/kD.
