Figure 3.

Infection with Anaplasma phagocytophilum leads to an enhanced release of LTB₄. Primary human neutrophil granulocytes were coincubated with cell-free A. phagocytophilum for 300 min and extracellular bacteria were removed by washing. (a) Immunocytochemical staining of coincubated PMN reveals the presence of intracellular bacteria (arrows). (b) A. phagocytophilum-infected and noninfected neutrophils were treated with ionomycin (0.2 μM, 10 min). LTB₄ content of the supernatants was measured by ELISA. n = 6, n.d.: not detectable, and ^∗p ≤ 0.05.