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. 2017 Oct 24;89(17):1821–1828. doi: 10.1212/WNL.0000000000004578

Figure 3. c.-271_-271delTCA reduces UFM1 promoter activity in SY-5Y neuroblastoma and U373 astroglioma cell lines.

Figure 3

(A–D) c.-273_-271delTCA in human UFM1 (hUFM1) promoter significantly reduces reporter gene expression in SY5Y (p = 0.001) and U373 (p = 0.014) but not in HeLA (cervix carcinoma) or HOG-F2 (oligodendrocytoma) cell lines. Promoter activity was measured with dual luciferase reporter assays. The nanoluciferase/firefly luciferase ratio measured in cells transfected with wildtype hUFM1 was arbitrarily set at 1 on the y-axis. delTCA indicates cells transfected with human UFM1 promoter harboring c.-273_-271delTCA. Data represent mean ± SD values obtained from 2 independent experiments testing the luciferase activity in duplo and were analyzed by 2-tailed t test.