Figure 4.

Silencing of the p65 subunit confirms results obtained with IT-901. (A) chronic lymphocytic leukemia (CLL) cell lines were infected with lentiviral particles containing plasmids coding for an shRNA scramble (Scr) or shRNA p65 (A and B). Expression of the NF-κB complex was checked by western blot and p50 and p65 expression quantified over actin, showing a significant reduction for p65, while for p50 statistical significance was not reached. Silencing of p65 diminished also the expression of the anti-apoptotic protein XIAP and of the full-length (FL) Caspase3 protein. Actin was used as a loading control. (B) Apoptosis analysis by flow cytometry of cell lines infected with shRNA lentiviral particles (n=5).