Table 1.
UCP-LF CAA assay formats for urine analysis
|
Assay
format |
Analyzed
volume (μl) |
TCA extraction solution
volume (μl) / % (w/v) |
Amicon (centrifugal)
concentration device |
QC threshold
1
singlet test (pg/ml) |
|---|---|---|---|---|
| UCAA10 | 10 μl | 10 μl / 4% | none | 10 |
| UCAA250 | 250 μl | 250 μl / 4% | 0.5 ml Filter Tube | 1 |
| UCAA2000 | 2 000 μl | 2 000 μl / 4% | 4 ml Filter Tube | 0.1 |
| UCAA14000 | 14 000 μl | 1 000 μl / 30%2 | 15 ml Filter Tube3 | 0.03 |
| UCAA40000 | 40 000 μl | 10 000 μl / 10%2 | 50 ml Cell3 | 0.01 |
1Quality control threshold (CAA concentration) for single test with wet reagents [20]
2Utilizing relevantly higher TCA concentration than the 4% (w/v) applied in the standard formats
3The 15 ml filter tube and 50 ml cell concentration is a two-step procedure requiring an additional 0.5 ml filter tube to concentrate down to 20 μl