Fig. 6.

Lotilaner antagonism is not affected by mutation causing dieldrin and fipronil resistance. a Current traces obtained from a X. laevis oocyte expressing DmR2-GABA receptors. The interrupted trace shows the fifths response to 1 μM GABA application representing the baseline. The black and grey traces (first and second recording, respectively) have been obtained after oocyte exposure to 1 μM GABA co-applied with 100 nM fipronill. The bars indicate the time period of GABA application (interrupted line) or GABA co-applied with fipronil (solide line). b Averaged inhibition concentration-response curves measured for dieldrin (black circle), fipronil (black triangle, dashed line) and lotilaner (black square), obtained from oocytes expressing DmR2-GABA. Individual curves were standardized to the fitted maximal current amplitude and subsequently averaged. Mean ± SEM of experiments carried out with at least four oocytes from two batches each is shown