Fig. 3.

Alignment of family 17 with the ML domain. Alignment of nine representative protein sequences of family 17 with nine representative sequences of the ML domain for different species (complete alignment in Additional file 3). The alignment starts 20 amino acids after the signal peptide and covers the entire protein length of both effector proteins and ML domain sequences. Two cysteines and a glycine are perfectly conserved (indicated with asterisks). Moreover, the spacing between hydrophobic amino acids is conserved throughout the alignment (the complete alignment is available in Additional file 3). B. graminis genes are named according to their f.sp. (Bg_POA = B.g. poae; Bgh = B.g. hordei; Bgt = B.g. tritici; Bg_AVE = B.g. avenae; Bg_LOL = B.g. infecting Lolium). The members of the ML domain are named with their Uniprot ID: GENENAME_SPECIES (ARATH = Arabidopsis tahliana; VITVI = Vitis vinifera; USTMA = Ustilago maydis; CRYNJ = Cryptococcus neoformans; PICGU = Meyerozyma guilliermondii; LODEL = Lodderomyces elongisporus; CANAL = Candida albicans; PICST = Scheffersomyces stipitis)