Figure 1.

NaStEP interacts with the pollen protein NaSIPP. Interaction between NaStEP and NaSIPP was detected by a yeast two-hybrid assay. A, Cotransformed S. cerevisiae growing on the quadruple dropout, or QDO, medium (synthetic dextrose [SD]/Trp-Leu-Ade-His). B, Yeast growth on QDO medium supplemented with 5-bromo-4-chloro-3-indolyl-α-d-galactopyranoside (X-α-Gal) and aureobasidin; positive interactions support growth and turn blue. C, Yeast growth on double dropout, or DDO, medium (SD/Trp-Leu). Full NaSIPP, The whole NaSIPP protein; Mir1, MPC of S. cerevisiae. BD and AD empty vectors were used as negative controls.