Figure 2.

NaSIPP is a phosphate transporter and partially complements the absence of Mir1 in S. cerevisiae. A, Growth curve of the yeast mutant Δmir1, the wild-type (WT) strain, and the Δmir1 yeast transformed with the plasmid pYES-DEST52 (empty vector) and with construct Pic2::pYES-DEST52, NaSIPP::pYES-DEST52, and Mir1::pYES-DEST52. Yeast were grown on liquid glycerol medium at 30°C. B, Yeast were grown on solid glycerol medium at 30°C for 10 d. C, Yeast replica plated on solid Glc medium were incubated for 3 d at 30°C.