Figure 3.

Characterization of GmSHMT08 mutants. A, SCN phenotype of GmSHMT08 mutants in comparison with resistant (cv Forrest and EXF67) and susceptible (cv Essex, EXF63, and EXF50) soybean lines. Each dot corresponds to the number of cysts on an individual plant. Asterisks denote statistically significant differences in resistance in comparison with cv Forrest (paired Student’s t test, P < 0.01, red; P < 0.0001, black). B, Rhg4 expression in cv Forrest and the F234 mutant. Root cDNA was used as a template for qPCR. C, E. coli complementation assay using mutant GmSHMT08 proteins. The proteins were expressed under the control of the IPTG-inducible T7 promoter in E. coli shmt mutant GS245 pLysS strain. Proteins were induced with 0.25 mm IPTG at 37°C. The absorbance of bacterial cultures at 600 nm was measured at the time intervals shown and plotted.