Figure 5.
drirD and overexpressing lines are more sensitive to ABA. A and B, Stomata of drirD and the overexpressing lines are more sensitive to ABA-induced stomata closure. A, Representative images of stomata on leaf epidermis in response to ABA treatment. The fifth and sixth rosette leaves of 4-week-old plants were incubated in a stomata open solution for 2 h and then transferred to the open solution supplemented with 20 µm ABA. Images were taken before and 2 h after ABA treatment. Bar = 100 µm. B, Stomata aperture (measured by width over length) in response to the ABA treatment in different genotypes. At least 500 stomata from 30 leaves of each genotype were measured. CK, control with no ABA treatment. *, P < 0.05 by Student’s t test compared with the wild type (WT). Error bars represent sd. C, Seedling sensitivity to ABA. Four-day-old seedlings of the wild type, drirD, A12, and A14 on 1/2 MS plates were transferred to 1/2 MS plates supplemented with 0, 2, or 5 µm ABA. Representative images show the morphology of seedlings 8 d after growth on ABA medium plates. D, Green leaf percentage of each genotype. At least 100 seedlings for each treatment were scored. *, Significant between the sample and the wild type at P < 0.05 by Student’s t test. Data represent means ± sd.