Figure 4.

Immunological analyses reveal detailed molecular glycan structures in Brachypodium root sections. A, Toluidine Blue was used to stain sections and identify cell types (Hardtke and Pacheco-Villalobos 2015). B, Immunolabeling of (arabino)xylan using CCRC-M148, CCRC-M154, and LM10 in root tips of Brachypodium. C, Putative AG side chains with expected binding of JIM14 and JIM16. D, Immunolabeling of AG structures using JIM14 and JIM16. Arabinofuranosidases and galactanases were incubated on the sections prior to the antibody staining to reveal xylan and galactan epitopes. Representative results of three independent biological replicates are shown. Bars = 50 µm.