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. 2017 Oct 31;175(3):1254–1268. doi: 10.1104/pp.17.00821

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Figure 4. — GUS staining of plants with YSL1 (A and B) and YSL3 (C and D) promoters fused to the GUS reporter gene. Mesophyll is indicated as m, and phloem is indicated as p. A, YSL1p::GUS rosette leaf shows YSL1 expression specific to vasculature tissues. B, Cross section of the midrib of YSL1p::GUS rosette leaf reveals darkest staining in the xylem parenchyma (xp). C, YSL3p::GUS rosette leaf shows YSL3 expression specific to the vasculature tissues. D, Cross section of the midrib of YSL3p::GUS rosette leaf reveals staining largely in the phloem. Phloroglucinol is used as a counterstain for lignified tissue.