Figure 5.

ZmTps21 transcripts are elicited by diverse fungi and precede β-costic acid accumulation detectable in diverse maize lines. A to D, Average (n = 4; ±se) Mo17 ZmTps21 (A), β-costic acid (B), ZmTps6/11 (C), and zealexin A1 (D) as quantitative reverse transcription (qRT)-PCR fold changes of transcripts and corresponding phytoalexin concentrations (μg g⁻¹ fresh weight [FW]) in intact control stems (Con) or those damaged and treated with either water (Dam) or a heat-killed Fusarium spp. elicitor (F.E.) hyphae preparation after 1, 2, or 4 d. E to H, Average (n = 4; ±se) Mo17 ZmTps21 (E), β-costic acid (F), ZmTps6/11 (G), and zealexin A1 (H) as qRT-PCR fold changes of transcripts and corresponding phytoalexin concentrations (μg g⁻¹ fresh weight) in intact control stems or those damaged and treated with either 100 μL of water alone or spore suspensions (1 × 10⁷ mL⁻¹) of R. microsporus (R.m.), A. parasiticus nor-1 (A.p.), F. verticillioides (F.v.), or C. heterostrophus (C.h.) and harvested at 2 and 4 d for transcripts and metabolites, respectively. I, Average (n = 4; ±se) β-costic acid concentrations (μg g⁻¹ fresh weight) in the scutella of 10-d-old maize seedlings from 15 inbred maize lines and mature field-collected roots displaying necrosis. Hybrids include sweet corn (variety Golden Queen [GQ]) and IBM-RIL-0287. Within plots, different letters (a–e) represent significant differences (all ANOVA, P < 0.05; Tukey’s test corrections for multiple comparisons, P < 0.05).