Figure 6.

A, Transcript levels of histone modification-related enzyme genes after treatment with H2O2. The transcript levels of all genes were measured by quantitative real-time PCR using mRNA prepared from normal and H₂O₂-treated maize aleurone layers. The x axis indicates time, and the y axis indicates relative expression values. The relative expression value of the control group (3 d) was defined as 1. The ubiquitin gene was used as an internal control. B, H₂O₂ did not affect H3K9ac, H4K5ac, and H3ac protein levels in the aleurone layers. Antibodies specific to H3K9ac, H4K5ac, and H3ac were employed to analyze histone extracts from aleurone layers treated with or without H₂O₂ for 3, 5, 7, and 9 d. Control, Distilled, deionized water; C, control; H, H₂O₂. Quantitative analysis of western blotting was performed using ImageJ. Error bars represent se (n = 3). No significant differences was observed, as compared with the Control group according to Student’s t test.