ChIP results of Cat-1. A, H3ac and H4K5ac levels in the promoter regions (sets A–E) and the exon (set F) of the Cat-1 gene after GA treatment in maize aleurone layers during the PCD process. The x axis indicates time, and the y axis indicates relative expression values. The relative expression value of the control group (set A) was defined as 1. The ubiquitin gene was used as an internal control. B, Schematic representation of the Cat-1 gene from −2,245 to +258 bp. Control, Distilled, deionized water. Each assay was repeated three times for every sample in three independent experiments. Error bars represent se (n = 3). #, P < 0.05 and *, P < 0.01 versus the control group according to Student’s t test.