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. 2017 Sep 21;175(3):1203–1219. doi: 10.1104/pp.17.00982

Figure 4.

Figure 4.

Expression profiles of selected genes from the microarray analysis tested by qRT-PCR. A, Expression changes of genes representative for distinct expression classes in wild-type light, pap7-1, and pap6-1 mutants compared to wild-type dark. Genes for light-harvesting complex II protein1.4, light harvesting complex II protein1.2, and “overexpression leads to pseudo-etiolation in light” represent genes displaying a reduced light induction in pap7-1 when compared to wild type. Genes encoding protein state transition kinase7, PGR5-like protein 1a, and σ-factor5 represent genes displaying no effect of pap7-1 on light induction, whereas the gene for protochlorophyllide oxidoreductase B represents an example for strong light repression both in wild type and the pap7-1 mutant. The genes for glutathione peroxidase 7 and (6 to 4) DNA photolyase were used as examples displaying light induction in wild type and further promotion in pap7-1. Genes for SEN1 and HFR1 senescence-associated protein DIN1 and the transcription factor Long Hypocotyl after far-red1 represent genes displaying repression in wild type but promotion in the mutant. As additional genetic control, a defect in the gene for a phospho-fructokinase like1 protein (pap6-1/fln1) was used to detect potential mutation-specific responses. Expression of both pap genes was tested in all RNA samples as further control. B, Difference in expression change between light-grown wild-type and the pap7-1 and pap6-1 mutants. Negative values indicate lower expression, positive values higher expression than in wild type. Data given represent means of three independent experiments. Primers used are given in Supplemental Table S4. LHCB1.2, light-harvesting complex II protein 1.2; LHCB1.4, light-harvesting complex II protein 1.4; STN7, state transition kinase7; PGRL1a, PGR5-like protein1a; PORB, protochlorophyllide oxidoreductase B; SIG5, σ-factor 5; ATGPX7, glutathione peroxidase7; oePEL, “overexpression leads to pseudo-etiolation in light”; UVR3, (6 to 4) DNA photolyase; SEN1, senescence-associated protein DIN1; HFR1, transcription factor Long Hypocotyl after far-red1; WT, wild type.