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. 2017 Sep 13;175(3):1158–1174. doi: 10.1104/pp.17.01035

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Figure 2. — Clustering analysis of time-course RNA-seq revealed five characteristic transcriptional changes induced by wounding. A, The 1,979 genes in cluster 1 are transiently induced within 1 h after wounding. GO categories such as response to stimuli and response to wounding are overrepresented. B, Cluster 2 contains 1,903 genes induced between 3 and 6 h after wounding. Genes involved in various metabolic processes are enriched in this cluster. C, Cluster 3 includes 3,854 genes induced after 3 h. GO categories such as gene expression, translation, and cellular biosynthetic processes are overrepresented. D, The 3,535 genes in cluster 4 are initially down-regulated but subsequently up-regulated after 12 h. Genes involved in the regulation of cell cycle are enriched in this cluster. E, Cluster 5 contains 3,334 genes that are down-regulated after wounding. Genes involved in stress response and cell wall biogenesis are overrepresented in this cluster. Magenta lines highlight the centroid of each cluster. Top 20 GO categories are shown with color codes representing the log10 (P value).