Figure 3.

Wound-induced JA synthesis and response are inhibitory for callus formation. A, Expression profiles retrieved from RNA-seq show that many genes involved in JA biosynthesis and response are induced within 1 h after wounding. Note that only genes with RPKM > 1 that change significantly along the time course are shown (20/27 genes for JA synthesis; 22/24 genes for JA responses). The color code indicates the log2 value of relative fold change compared to 0 h. B, Overall levels of endogenous JA are increased transiently after wounding. Alphabetical letters indicate statistical significance determined by ANOVA and Tukey’s multicomparison test (n = 3; P < 0.01). C, Callus induction is enhanced in aos and coi1-16B compared to wild type (Col-gl). Asterisks indicate statistical significance determined by proptest (n > 200 for each genotype; P < 0.05).