ABA-mediated signaling is inhibitory for callus formation at wound sites. A, Expression profiles retrieved from RNA-seq datasets show that a subset of ABA biosynthesis and signaling genes are induced within 24 h after wounding. Note that only genes with RPKM > 1 that change significantly along the time course are shown (9/15 genes for ABA synthesis; 37/41 genes for ABA responses). B, Overall endogenous ABA content gradually decreases after wounding. Alphabetical letters indicate statistical significance determined by ANOVA and Tukey’s multicomparison test (n = 3; P < 0.01). C, Callus formation tends to be enhanced in both ABA synthesis mutants, i.e. nced3-2, aba2-1, aba2-2, aba3-1, and aao3-4, and signaling mutants, i.e. abi1-1. Asterisks indicate statistical significance determined by proptest (n > 100 for each genotype; P < 0.05).