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. 2017 Sep 13;175(3):1158–1174. doi: 10.1104/pp.17.01035

Figure 6.

Figure 6.

Wounding does not modify overall auxin biosynthesis and signaling. A, Expression profiles retrieved from RNA-seq datasets show that the expression of auxin biosynthesis genes YUC5 and TAA1 are induced after wounding while other biosynthesis genes, YUC2, YUC6, and YUC9, are repressed. A subset of auxin response genes is transiently up-regulated at 1 h after wounding, but others remain unchanged or downregulated. Note that only genes with RPKM > 1 that change significantly along the time course are shown (5/13 genes for IAA synthesis; 24/50 genes for IAA responses). B, An overall content of endogenous IAA level does not change significantly after wounding (n = 3; P = 0.273; ANOVA). C, Time-course imaging of DR5rev:GFP plants shows that auxin response is not elevated at wound sites of hypocotyl explants. Dashed lines indicate wound sites. The strong DR5rev:GFP expression in root tips confirms the reporter activity. Scale bar = 0.5 mm.