Expression patterns of OsmiR408 and OsUCL8, and subcellular localization of the OsUCL8 protein. A, Relative expression patterns of OsmiR408 and OsUCL8 in different tissues. Values are expressed as means ± sd. B, Spatial expression pattern analysis of OsmiR408 and OsUCL8 in young panicles and grains by GUS staining. Bars = 2 mm. C, In situ hybridization of OsmiR408 and OsUCL8 during panicle development in wild-type (WT) plants and in OXmiR408 plants. Bars = 100 µm. D, Subcellular localization of OsUCL8. The empty vector with or without ATG before the GFP gene was used as a positive or negative control, respectively. BF, Bright field. Bars = 10 µm.