Table II. Lignin components released from cell walls from the two tissue regions and quantified by DFRC and DFRC-Pr.
Masses were converted to the native Mr values and not the acetylated/hydrogenated Mr values detected by gas chromatography (GC)-MRM-mass spectrometry (MS). All values are reported here as mg g−1 EL (μmol g−1 EL) ± se. N/D, Not detected; –, not applicable.
| Component | DFRC |
DFRC-Pr |
Applying ML-OH-to-ML-Ac Ratio to the DFRC Results |
|||
|---|---|---|---|---|---|---|
| Outer Tissue | Inner Tissue | Outer Tissue | Inner Tissue | Outer Tissue | Inner Tissue | |
| H-OH/Aca | 2.4 ± 0.1a (10.1 ± 0.5) | 1.9 ± 0.1a (8.1 ± 0.2) | – | – | – | – |
| G-OH/Aca | 39.6 ± 1.0a (150 ± 4) | 45.4 ± 4.5a (172 ± 17) | – | – | – | – |
| S-OH/Aca | 107 ± 3a (365 ± 9) | 143 ± 5a (487 ± 16) | – | – | – | – |
| H-OHb | – | – | 1.0 ± 0.1 (6.8 ± 0.9) | 1.0 ± 0.0 (6.4 ± 0.2) | 1.5 ± 0.1 (10.1 ± 0.5) | 1.2 ± 0.0 (8.1 ± 0.2) |
| G-OHb | – | – | 14.3 ± 0.2 (79.6 ± 1.2) | 12.9 ± 0.3 (71.8 ± 1.9) | 21.1 ± 0.5 (117 ± 3) | 25.4 ± 2.5 (141 ± 14) |
| S-OHb | – | – | 18.2 ± 0.3 (86.6 ± 1.3) | 17.0 ± 2.1 (80.9 ± 10) | 28.4 ± 0.7 (135 ± 3) | 37.9 ± 1.3 (180 ± 6) |
| H-Acc | – | – | – | – | – | – |
| G-Acc | – | – | 5.1 ± 1.5 (23.1 ± 6.6) | 3.5 ± 0.0 (15.6 ± 0.1) | 7.3 ± 0.2 (33 ± 1) | 6.9 ± 0.7 (31 ± 3) |
| S-Acc | – | – | 38.9 ± 6.1 (154 ± 24) | 34.1 ± 2.1 (135 ± 8) | 58.0 ± 1.5 (230 ± 6) | 77.3 ± 2.6 (307 ± 10) |
| G-BA | 0.2 ± 0.0 (0.8 ± 0.1) | 1.0 ± 0.1 (3.6 ± 0.3) | – | – | 0.2 ± 0.0 (0.8 ± 0.1) | 1.0 ± 0.1 (3.6 ± 0.3) |
| S-BA | 10.2 ± 0.3 (33 ± 1) | 30.7 ± 4.4 (98 ± 14) | – | – | 10.2 ± 0.3 (33 ± 1) | 30.7 ± 4.4 (98 ± 14) |
| G-pBA | 3.2 ± 0.2 (10.6 ± 0.8) | 3.7 ± 0.2 (12.5 ± 0.7) | – | – | 3.2 ± 0.2 (10.6 ± 0.8) | 3.7 ± 0.2 (12.5 ± 0.7) |
| S-pBA | 42.7 ± 3.4 (129 ± 10) | 69.1 ± 1.2 (209 ± 3) | – | – | 42.7 ± 3.4 (129 ± 10) | 69.1 ± 1.2 (209 ± 3) |
| G-VA | 0.01 ± 0.00 (0.02 ± 0.00) | 0.01 ± 0.00 (0.03 ± 0.00) | – | – | 0.01 ± 0.00 (0.02 ± 0.00) | 0.01 ± 0.00 (0.03 ± 0.00) |
| S-VA | 0.15 ± 0.01 (0.40 ± 0.03) | 0.22 ± 0.01 (0.62 ± 0.04) | – | – | 0.15 ± 0.01 (0.40 ± 0.03) | 0.22 ± 0.01 (0.62 ± 0.04) |
| G-pCA | 0.04 ± 0.00 (0.12 ± 0.00) | 0.01 ± 0.00 (0.02 ± 0.00) | – | – | 0.04 ± 0.00 (0.12 ± 0.00) | 0.01 ± 0.00 (0.02 ± 0.00) |
| S-pCA | 5.53 ± 0.01 (15.5 ± 0.0) | 0.86 ± 0.01 (2.4 ± 0.0) | – | – | 5.53 ± 0.01 (15.5 ± 0.0) | 0.86 ± 0.01 (2.4 ± 0.0) |
| G-FA | N/D | N/D | – | – | N/D | N/D |
| S-FA | 0.01 ± 0.00 (0.02 ± 0.00) | 0.02 ± 0.00 (0.05 ± 0.01) | – | – | 0.01 ± 0.00 (0.02 ± 0.00) | 0.02 ± 0.00 (0.05 ± 0.01) |
a
Released by DFRC and reported here as the monolignol 4,9-diacetate.
b
Released by DFRC-Pr as the monolignol 4,9-dipropionate; reported as the 4,9-dihydroxy-monolignol.
c
Released by DFRC-Pr as the monolignol 4-propionate-9-acetate; reported as the 4-hydroxy-monolignol 9-acetate.