Figure 3.

DCL2 enhances the biogenesis of RDR6-dependent secondary siRNAs. GFP-specific siRNA profiles are shown in roots of 10027-3 wild-type (WT), dcl2 (Kas-1), and dcl4-5 seedlings. All 10027-3 lines were homozygous for the 10027-3 T-DNA locus. Plants were grown in vitro under long days for 1 week. Positive and negative scales on the y axis represent sense and antisense siRNA profiles, respectively. The x axis shows nucleotide positions in the GFP coding sequence and 3′ UTR. Sequences homologous to the GF-specific dsRNA expressed specifically in the root tip to initiate systemic PTGS are indicated in darker gray. The data presented are averages of two or three biological replicates that are shown individually in Supplemental Figure S9.