Figure 1.

LjNPF8.6 transcriptional regulation. A, Expression in different organs. RNAs were extracted from wild-type plants grown on Gamborg B5 derivative medium without N source at 4 weeks post inoculation (wpi). Mature flowers were obtained from L. japonicus plants propagated in the growth chamber. B, Time course of expression in root and nodule tissues after M. loti inoculation. RNAs were extracted from roots of wild-type seedlings grown in N starvation conditions at different times after inoculation (R0, 24 h, and 72 h) and from young (10 d post inoculation) and mature (28 d post inoculation) nodules. Expression levels are normalized with respect to the internal control ubiquitin (UBI) gene and plotted relative to the expression of flowers (A) and R0 (B). White bars, LjNPF8.6; gray bars, LjNIN. Data bars represent means and sd of data obtained with RNA extracted from three different sets of plants and three real-time PCR experiments.